Archives

  • 2018-07
  • 2019-04
  • 2019-05
  • 2019-06
  • 2019-07
  • 2019-08
  • 2019-09
  • 2019-10
  • 2019-11
  • 2019-12
  • 2020-01
  • 2020-02
  • 2020-03
  • 2020-04
  • 2020-05
  • 2020-06
  • 2020-07
  • 2020-08
  • 2020-09
  • 2020-10
  • 2020-11
  • 2020-12
  • 2021-01
  • 2021-02
  • 2021-03
  • 2021-04
  • 2021-05
  • 2021-06
  • 2021-07
  • 2021-08
  • 2021-09
  • 2021-10
  • 2021-11
  • 2021-12
  • 2022-01
  • 2022-02
  • 2022-03
  • 2022-04
  • 2022-05
  • 2022-06
  • 2022-07
  • 2022-08
  • 2022-09
  • 2022-10
  • 2022-11
  • 2022-12
  • 2023-01
  • 2023-02
  • 2023-03
  • 2023-04
  • 2023-05
  • 2023-06
  • 2023-08
  • 2023-09
  • 2023-10
  • 2023-11
  • 2023-12
  • 2024-01
  • 2024-02
  • 2024-03
  • 2024-04

    • Trelagliptin In conclusion our studies identified the dnmt g

    2020-08-04

    In conclusion, our studies identified the dnmt3 gene sequence and characterized the role of this gene in regulating reproductive fertility in fish. Dnmt3 was under E2 control and its regulation in meiotic pachytene impairment of oocytes in 3n fish might be correlated with the exon CGI methylation and gene expression of msh4. Our results elucidated the epigenetic mechanism of dnmt3 regulating sterility in female 3n fish for the first time, and played a significant role in reproductive biology in theory and in aquaculture breeding in practice.
    Author contributions
    Competing interests
    Acknowledgements Our work was supported in whole or part by the National Natural Science Foundation of China, China (31402297, 31430088, 31702328); the Earmarked fund for China Agriculture Research System, China (CARS-45); Natural Science Foundation of Hunan Province, China Grants (2018JJ3338); Hunan Provincial Natural Science and Technology Major Project, China (2017NK1031); Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province, China (20134486).
    Introduction DNA methyltransferase (MTase) is a primary epigenetic modification enzyme that catalyzes the DNA methylation by transferring a methyl group to the target Trelagliptin or cytosine residue from donor S-adenosyl-L-methionine (SAM) [1], [2], [3], [4]. It plays an essential role in regulating gene expression and maintaining genomic stability [5], [6], [7]. Abnormal DNA MTase activity is strongly associated with tumorigenesis by silencing transcription of gene expression and inactivating tumor suppressor gene [8], [9]. Also, studies show that DNA MTase activity is directly linked with DNA methylation level, and the DNA methylation level can evince the abnormality much earlier than other signs of malignancy [10]. DNA MTase has thus been considered as a promising biomarker for cancer diagnosis and a potential therapeutic target for cancer therapy [11], [12], [13], [14]. DNA MTase activity detection in a sensitive and specific way should contribute to resolving its related function in cancer initiation and exploring its possible application in cancer theranostics. The methods for DNA MTase activity assay involve radioactive labelling [15], gel electrophoresis [16], high-performance liquid chromatography [17], [18], bisulfite conversion [19], [20], and affinity enrichment [21], [22]. In these methods, the methylated bases under the action of active MTase could change the DNA\'s migration rate, hybridization efficiency, or affinity to antibodies, so as to generate the signal different from that under the action of inactive MTase. Recently, the methods based on the MTase-dependent DNA probe cleavage have been proposed [23]. In these methods, the methylated bases can facilitate or inhibit the probe cleavage by the restriction endonuclease that results in the release of the oligonucleotide fragment. The oligonucleotide fragment can be readily modified, flexibly designed and further adopted for the downstream reaction, offering more effective alternatives for signal transduction and amplification. Initially, the released oligonucleotide fragment directly generates the signal [24], [25], [26], [27], [28], [29], such as the fluorescence caused by fluorescence resonance energy transfer change [24], [25], [26], [27] or the electrochemical current caused by electron transfer efficiency change [28], [29]. It achieves a simple DNA MTase activity detection but the sensitivity is compromised due to the limited amount of released oligonucleotide fragments under the action of MTase. Subsequently, the released oligonucleotide fragment is utilized as trigger for the cascade amplification [30], [31], [32], [33], [34], [35], [36], [37], [38], [39], [40], [41], [42], such as acting as DNAzymes for the catalysis of substrate hydrolysis [30], [31], templates for strand displacement amplification [32], [33] or auxiliary strands for nuclease-mediated cyclic digestion of reporter DNA [34], [35]. Although the sensitivity is improved, in most of these reported methods, only one trigger for cascade amplification is released from the DNA probe under the action of MTase. It means that for each DNA probe, the MTase recognition event is converted to merely single trigger-induced signal transduction, still restricting the further increase of sensitivity. In the view of that more sensitive MTase activity detection is important to the MTase-based early diagnosis [43], new detection strategies are still needed to be developed.